Buldugum bazi agar tariflerini buraya eklemek istiyorum.
All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure.
500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.
Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)
Amaranth Soy Agar
20 grams amaranth flour
20 grams soy flour
9 grams agar agar
500 ml potable or distilled water
EntheoGenesis No.442
10 grams amaranth flour
10 grams brown rice flour
10 grams potato flour
10 grams soy flour
2 grams malted barley
9 grams agar agar
500 ml potable or distilled water
Oatmeal Neopeptone Agar
40 grams oatmeal or oat flour
2 grams neopeptone (optional)
9 grams agar agar
500 ml potable or distilled water
Modified Sabouraud's Medium
25 grams barley flour
5 grams dextrose
2 grams neopeptone (optional)
1 gram yeast extract
9 grams agar agar
500 ml potable or distilled water
Cornmeal Dextrose Agar
25 grams yellow cornmeal
3 grams dextrose
9 grams agar agar
500 ml potable or distilled water
Barley Malt Extract Agar
40 grams barley flour
2 grams malt extract
1 gram yeast extract (optional)
9 grams agar agar
500 ml potable or distilled water
Dr. Pollock's Modified Agar
10 grams dried dog food (ground to flour)
10 grams amaranth flour
2 grams dextrose or malt extract
9 grams agar agar
500 ml potable or distilled water
All recipes call for 500ml distilled water for 1/2 liter agar.
If you use water from the tap make sure to boil it first to get rid of the chlorine. The chlorine in tap water much like the food preservatives mentioned earlier is also not good for your cultures.
For 1 liter recipes double the agar, double the nutrients, i.e. 20 grams agar, 20 grams nutrient source.
LMEA (Light Malt Extract Agar)
10 grams light malt extract
10 grams agar agar
MYA (Malt Yeast Agar)
2 grams brewers yeast
10 grams malt extract
10 grams agar agar
DFA (dog food agar)
Boil 1/2 cup dry dog food in 500ml water. Strain the solution and add 10 grams agar powder.
OVA (ovaltine agar)
10 grams ovaltine
10 grams agar agar
FFA (fish food agar)
10 grams fish food flakes
10 grams agar agar
RFA (rabbit food agar)
10 grams rabbit food pellets
10 grams agar agar
PDA (potato dextrose agar)
Take 300 grams of potatoes. Cut them and boil them in 1/2 gallon H2O for 30 minutes on a simmer. The liquid will reduce to ~ 1 litre in that amount of time.
Take the liquid and strain it out. The starches in the liquid are what you need so toss the potatoes out or make a snack with them,
Use 500 ml of the potato water as the H2O source w/
10 grams Dextrose
10 grams agar agar
Pleurotus ostreatus
Suggested Agar Culture Media: Malt Yeast Peptone Agar (MYPA), Potato Dextrose Yeast Agar (PDYA), Oatmeal Yeast Agar (OMYA), or Dogfood Agar (DFA). Optimal growth seen at pH 5.5-6.5.
Pleurotus eryngii
Suggested Agar Culture Media: Malt Yeast Peptone Agar (MYPA) or Potato Dextrose Yeast Agar (PDYA).